Coding

Part:BBa_K1813009

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-27)

nicF

nicF Gen Bank accession number Q88FY5.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 439
  • 1000
    COMPATIBLE WITH RFC[1000]

Background of nicF

Part Description

Codon optimized coding sequence of nicF, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813017 and BBa_K1813023 .

Part Origin

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function

The enzyme coded for by nicF converts Maleamic Acid to Maleic Acid.

References

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

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Parameters
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